A Proposal to Test the Effects of Factor ECAT1 on Pluripotency, from Reprogramming to Differentiation of Human Somatic Cells
نویسنده
چکیده
.......................................................................................................................... 5 Introduction .................................................................................................................... 6 The Discovery of the Stem Cell .............................................................................................. 6 Debating Embryonic Stem Cells ............................................................................................. 7 Stem cell characteristics ........................................................................................................ 8 Alternative techniques .......................................................................................................... 9 Inducing Pluripotency: the 2006 experiment ...................................................................... 10 Oct3/4 .............................................................................................................................. 11 Sox2 ................................................................................................................................. 11 Klf4 ................................................................................................................................... 12 c-Myc ............................................................................................................................... 12 Inducing Pluripotency: the 2007 human experiment .......................................................... 13 Difficulties with iPSCs .......................................................................................................... 14 Proposal ........................................................................................................................ 16 ECAT1................................................................................................................................... 16 Proposed Experiments ......................................................................................................... 17 Materials & Methods ..................................................................................................... 19 Stage 1: Retroviral transduction-based reprogramming .................................................... 19 Confirming pluripotency .................................................................................................. 21 Stage 2: hiPSCs vs hESCs ...................................................................................................... 22 Molecular comparisons ................................................................................................... 22 Genomic comparisons ..................................................................................................... 23
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